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Sudan Black B Dye (Histology Stain)


Sudan Black B Basic Facts:
  • Sudan Black B has the following formula - C26H24N4O (pictured).
  • Other names - Fat Black HB.
  • Dye class - Azo.
  • It is a fat-soluble (lysochrome) diazo dye.
  • Dark brown to black powder.
  • Maximum absorption at 596-605 nm.
  • Melting point 120-124 °C.
  • Insoluble in water.
  • Moderately soluble in ethanol.
Sudan Black B Actions:
  • Histologically used for staining of neutral triglycerides & lipids on frozen sections.
  • Also stains some lipoproteins on paraffin sections.
  • Stains structures blue-black.
Sudan Black B Other Features:
  • One of the dyes used for Sudan staining.
  • Other similar dyes include Oil Red O, Sudan III, & Sudan IV.
  • Can stain other materials, as it is not so specific for lipids as other sudan dyes.
  • Sudan Black B can also be used for fingerprint enhancement.
Clinical Facts:
  • Sudan Black stains lipofuscins (wear-and-tear pigments).
  • Helps in differentiating haematological disorders.
  • Sudan black stains myeloblasts, but not lymphoblasts.
  • Thus helps in identification of specific leukemias, particularly acute myelocytic leukemia.

Posted by ALCHEssMIST.
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