Sudan Black B Basic Facts:
- Sudan Black B has the following formula - C26H24N4O (pictured).
- Other names - Fat Black HB.
- Dye class - Azo.
- It is a fat-soluble (lysochrome) diazo dye.
- Dark brown to black powder.
- Maximum absorption at 596-605 nm.
- Melting point 120-124 °C.
- Insoluble in water.
- Moderately soluble in ethanol.
- Histologically used for staining of neutral triglycerides & lipids on frozen sections.
- Also stains some lipoproteins on paraffin sections.
- Stains structures blue-black.
- One of the dyes used for Sudan staining.
- Other similar dyes include Oil Red O, Sudan III, & Sudan IV.
- Can stain other materials, as it is not so specific for lipids as other sudan dyes.
- Sudan Black B can also be used for fingerprint enhancement.
- Sudan Black stains lipofuscins (wear-and-tear pigments).
- Helps in differentiating haematological disorders.
- Sudan black stains myeloblasts, but not lymphoblasts.
- Thus helps in identification of specific leukemias, particularly acute myelocytic leukemia.
Posted by ALCHEssMIST.
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